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Free Rad Biol Med 2002

Of note, the commercial Iscoves Modified Dulbeccos Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 M as compared to physiological plasma concentration of 4075 M cysteine in SCD patients, which decreased further to 3060 M during vaso-occlusive pain episodes 36

Liston, S

To enhance the sensitivity of detection of the diagnostic products, instead of IAM we used a triphenylphosphonium cation (TPP + ) derivative of iodoacetamide (IAM-TPP) 40

The role of glutathione in disulphide bond formation and endoplasmic-reticulum-generated oxidative stress